GSM1506076: CHD8 Novus60417; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CHD8
Cell type
Cell type Class
Pluripotent stem cell
Cell type
iPSC derived neural cells
NA
NA
Attributes by original data submitter
Sample
source_name
human control NPCs
cell type
iPSC (GM8330-8)-derived NPC
passages
11
shRNA target gene
GFP
chip antibody
CHD8 (Novus Biological NB100-60417)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Complexes were precipitated with Dynabeads Protein A beads (Invitrogen), and immunoprecipitated chromatin was eluted in elution buffer de-crosslinked at 65°C for 8 hr (or O/N), and treated with proteinase K (Roche). DNA was purified by extracting with phenol and chloroform and precipitating in ethanol. Library preparation was carried out using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB Cat#E73705), according to the manufacturer's instructions.