Antigen

Antigen Class

TFs and others

Antigen

Spi1

Cell type

Cell type Class

Blood

Cell type

B cells

NA

NA

Sample

source_name

Splenic B cells, PU.1 KI, PU.1 IP

genotype

PU.1[155-/155-]

cell isolation

Splenic B cells

condition

Ex-vivo

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions. Libraries were sequenced on the Genome Analyzer Iix following the manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

11719603

Reads aligned (%)

93.8

Duplicates removed (%)

22.2

Number of peaks

707 (qval < 1E-05)

mm9

Number of total reads

11719603

Reads aligned (%)

93.5

Duplicates removed (%)

22.5

Number of peaks

577 (qval < 1E-05)