Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Epidermis

Cell type

Dermal fibroblast

NA

NA

Sample

source_name

HG fibroblasts_INPUT

cell type source

HGPS patient 2 (TRS-HGPS)

cell type

HGPS dermal fibroblasts transformed by retroviral introduction of TERT, HRAS and SV40 T antigens (TRS-HGPS1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated cells and histone-DNA complexes were isolated with antibody. Library construction was performed following Illumina TruSeq 2.0 protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

41986781

Reads aligned (%)

97.9

Duplicates removed (%)

2.0

Number of peaks

1469 (qval < 1E-05)

hg19

Number of total reads

41986781

Reads aligned (%)

97.0

Duplicates removed (%)

2.3

Number of peaks

568 (qval < 1E-05)