Antigen

Antigen Class

TFs and others

Antigen

BRD4

Cell type

Cell type Class

Epidermis

Cell type

Dermal fibroblast

NA

NA

Sample

source_name

WT fibroblasts_BRD4_ChIPSeq

cell type source

age-matched control wild-type individual 1 (TRS-WT)

cell type

WT dermal fibroblasts transformed by retroviral introduction of TERT, HRAS and SV40 T antigens (TRS-WT1)

chip antibody

BRD4

chip antibody vendor

Bethyl labs

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated cells and histone-DNA complexes were isolated with antibody. Library construction was performed following Illumina TruSeq 2.0 protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

40215721

Reads aligned (%)

96.4

Duplicates removed (%)

64.5

Number of peaks

758 (qval < 1E-05)

hg19

Number of total reads

40215721

Reads aligned (%)

95.3

Duplicates removed (%)

65.3

Number of peaks

376 (qval < 1E-05)