Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
msl-1

Cell type

Cell type Class
Larvae
Cell type
Salivary glands
NA
NA

Attributes by original data submitter

Sample

source_name
salivary glands
developmental stage
third instar
Sex
male
chip antibody
polyclonal MSL1 antibody raised against the N-terminus (AA1-584)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was obtained from male 3rd instar larvae salivary glands as previously described (PMID: 16547175). In brief, collected salivary glands (10 per ChIP) were fixed, sonicated for 8 min (pulsed eight times for 30 sec, paused for 30 sec, high) in a Bioruptor (Cosmo Bio), and chromatin immunoprecipitated with the respective antibody. Libraries were prepared according to Illumina's instructions

Sequencing Platform

instrument_model
Illumina Genome Analyzer

dm3

Number of total reads
17762885
Reads aligned (%)
56.7
Duplicates removed (%)
29.0
Number of peaks
8634 (qval < 1E-05)

dm6

Number of total reads
17762885
Reads aligned (%)
55.1
Duplicates removed (%)
31.1
Number of peaks
7358 (qval < 1E-05)

Base call quality data from DBCLS SRA