Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Pc

Cell type

Cell type Class
Adult
Cell type
Temperature sensitive cells
NA
NA

Attributes by original data submitter

Sample

source_name
EZ2-2 ; E(z) temperature sensitive cells
cell line
EZ2-2 ; E(z) temperature sensitive cells
growth condition
grown at 25C
chip antibody
Pc (Pirrotta Lab; affinity purified)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Live cells were crosslinked with 1.8% formaldehyde for 10 min at 25°C and soluble chromatin prepared as described in Schwartz et al., 2006. Libraries were prepared according to Illumina's instructions from TruSeq ChIP Sample Preparation Kit with some modifications. Indexed adaptors from TruSeq ChIP Sample Prep Kit were used for barcoding. After adapter ligation, 250-500bp size was selected by gel size selection, and the subsequent amplification was done with 15 cycles. With the constructed libraries, 100bp paired-end sequencing was performed by Illumina HiSeq technology

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
9648110
Reads aligned (%)
170.6
Duplicates removed (%)
13.4
Number of peaks
5679 (qval < 1E-05)

dm6

Number of total reads
9648110
Reads aligned (%)
168.8
Duplicates removed (%)
14.0
Number of peaks
5948 (qval < 1E-05)

Base call quality data from DBCLS SRA