Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H1

NA

NA

Sample

source_name

H1

antibody

input

cell line

H1

cell type

human neural progenitor cells

genotype

WT

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, 1×107 cells of each sample were crosslinked in 1% formaldehyde with rotation for 10 minutes at room temperature. And then these crosslinked reactions were stopped by 0.125 M glycine with rotation for 5 minutes at room temperature. and cell sample were sonicated, and then ChIPed with FLAG M2-beads. DNA libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

47282607

Reads aligned (%)

99.2

Duplicates removed (%)

9.9

Number of peaks

1434 (qval < 1E-05)

hg19

Number of total reads

47282607

Reads aligned (%)

98.3

Duplicates removed (%)

11.4

Number of peaks

1185 (qval < 1E-05)