Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
Neuroepithelial-like stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
NESCs
sample type
ESC-derived neural precursors
cell type
human neuroepithelial-like stem cells (NESCs)
chip antibody
none (chromatin processed without incubation with the antibody)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin preparation and processing was performed starting from pellets of 5 106 NESCs and following the truChIP Chromatin Shering Kit standard protocol (PN520076, Covaris Inc.). Libraries were prepared according to Illumina's instructions accompanying the standard protocol of Illumina ChIP-seq DNA sample preparation kit (IP-102-1001, Illumina Inc.).

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg38

Number of total reads
32532250
Reads aligned (%)
96.8
Duplicates removed (%)
4.1
Number of peaks
1398 (qval < 1E-05)

hg19

Number of total reads
32532250
Reads aligned (%)
96.3
Duplicates removed (%)
5.1
Number of peaks
1524 (qval < 1E-05)

Base call quality data from DBCLS SRA