Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Breast
Cell type
3134
Tissue
Mammary
Lineage
cellLine
Description
Mammary

Attributes by original data submitter

Sample

source_name
GR ChIP-Seq input 60 min Cort Constant
cell line
3134
cell type
mouse mammary cells
treated with
600 nM Cort for 60 min

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and GR-DNA complexes, Pol II-DNA complexes or CTCF-DNA complexes were isolated with antibody. NEB Chip-Seq Prep Kit

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
34943592
Reads aligned (%)
96.7
Duplicates removed (%)
16.6
Number of peaks
473 (qval < 1E-05)

mm9

Number of total reads
34943592
Reads aligned (%)
96.4
Duplicates removed (%)
16.6
Number of peaks
569 (qval < 1E-05)

Base call quality data from DBCLS SRA