Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MAZ

Cell type

Cell type Class
Adipocyte
Cell type
T778
NA
NA

Attributes by original data submitter

Sample

source_name
T778 cells
differentiation stage
Liposarcoma cells
genotype
Control
passages
17-20
chip antibody
MAZ

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Metzger, E., et al. Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulation. Nat Cell Biol 10, 53-60 (2008). Libraries were prepared from immunoprecipitated DNA according to standard methods. ChIP-seq libraries were sequenced using a HiSeq 2000 (Illumina) and mapped to the hg19 reference genome using bowtie 2 Langmead, B., Trapnell, C., Pop, M. & Salzberg, S.L. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol 10, R25 (2009).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
50061272
Reads aligned (%)
66.6
Duplicates removed (%)
30.8
Number of peaks
26818 (qval < 1E-05)

hg38

Number of total reads
50061272
Reads aligned (%)
67.0
Duplicates removed (%)
30.6
Number of peaks
26074 (qval < 1E-05)

Base call quality data from DBCLS SRA