Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Adipocyte

Cell type

T778

NA

NA

Sample

source_name

T778 cells

differentiation stage

Liposarcoma cells

genotype

Control

passages

17-20

chip antibody

None

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Metzger, E., et al. Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulation. Nat Cell Biol 10, 53-60 (2008). Libraries were prepared from immunoprecipitated DNA according to standard methods. ChIP-seq libraries were sequenced using a HiSeq 2000 (Illumina) and mapped to the hg19 reference genome using bowtie 2 Langmead, B., Trapnell, C., Pop, M. & Salzberg, S.L. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol 10, R25 (2009).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

68313347

Reads aligned (%)

95.6

Duplicates removed (%)

6.2

Number of peaks

1262 (qval < 1E-05)

hg19

Number of total reads

68313347

Reads aligned (%)

95.0

Duplicates removed (%)

7.3

Number of peaks

1242 (qval < 1E-05)