Lysates were clarified from sonicated nuclei and MEF2A-DNA complexes were isolated with antibody. Libraries were prepared according to Rhee and Pugh (2011). Briefly, after ChIP, P2 adapters were ligated to DNA fragments using T4 ligase. Lambda exonuclease was added to digest unbound DNA. Samples were eluted and sequences were extended with phi29 polymerase. P2 adapters, specific to the 5' end were added. Samples were purified, run on a gel, and samples between 120-160 bp were selcted for sequencing. Libraries were sequenced on the Illumina sequencer following the manufacturer's protocol.