Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
JUNB

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
E5h_GFP_JunB_060619_with_flyDNA
cell type
Primary naive CD4 T cells from peripheral blood
treatment
Act. 5 h with anti-CD3 and -CD28 antibodies
passages
Primary
donor
healthy donor
chip antibody
JUNB Cell Signaling #3753
biowardrobe id
8043

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was immunoprecipitated with antibody and tagmented with nextera transposome (illumina) according to the ChIPmentation protocol in Schmidl et al (PBMD: 26280331). Libraries were prepared by chipmentation.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
12928959
Reads aligned (%)
95.2
Duplicates removed (%)
72.2
Number of peaks
282 (qval < 1E-05)

hg19

Number of total reads
12928959
Reads aligned (%)
93.9
Duplicates removed (%)
73.6
Number of peaks
272 (qval < 1E-05)

Base call quality data from DBCLS SRA