Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOS

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
E5h_GFP_cFOS_060619_with_flyDNA
cell type
Primary naive CD4 T cells from peripheral blood
treatment
Act. 5 h with anti-CD3 and -CD28 antibodies
passages
Primary
donor
healthy donor
chip antibody
cFOS Santa Cruz Biotechnology #sc-7202
biowardrobe id
8041

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was immunoprecipitated with antibody and tagmented with nextera transposome (illumina) according to the ChIPmentation protocol in Schmidl et al (PBMD: 26280331). Libraries were prepared by chipmentation.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
11474056
Reads aligned (%)
30.4
Duplicates removed (%)
68.1
Number of peaks
238 (qval < 1E-05)

hg38

Number of total reads
11474056
Reads aligned (%)
32.0
Duplicates removed (%)
66.8
Number of peaks
246 (qval < 1E-05)

Base call quality data from DBCLS SRA