Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYC

Cell type

Cell type Class
Neural
Cell type
NB69
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
neuroblastoma-derived cell line
growth media
RPMI 1640, 10% FBS, 1% Penicillin/Streptomycin, 2mM L-Glutamine
cell line
NB69
mycn status
non-amp
chip antibody
C-MYC (Santa Cruz N262, #sc-764)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked, flash frozen, and sent to Active Motif. According to Active Motif protocol, cells were lysed, sonicated, and then DNA-protein complexes were isolated with antibodies. Samples were reverse-crosslinked and DNA was purified. Illumina libraries were prepared by Active Motif using standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. After 15 cycles of PCR amplification, the resulting DNA libraries were quantified and sequenced on Illumina NextSeq 500.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
36915240
Reads aligned (%)
78.6
Duplicates removed (%)
18.9
Number of peaks
14910 (qval < 1E-05)

hg38

Number of total reads
36915240
Reads aligned (%)
80.1
Duplicates removed (%)
17.8
Number of peaks
15111 (qval < 1E-05)

Base call quality data from DBCLS SRA