GSM1498909: H3K4me1 ChIPSeq-KO2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me1
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
KO2-Late G1
cell line
Fucci human embryonic stem cell
antibody
H3K4me1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, lysates were isolated from sonicated nuclei and histone-DNA complexes were purified with antibody. For 4C, lysates were purified from restriction digested nuclei. ChIP-seq libraries were prepared by HudsonAlpha by first performing end-repair and adapter ligation of Chip'd DNA fragments, followed by size selection and PCR amplifcation to generate the library for sequencing. Library was prepared using the Illumina DNA Library Construction Kit. DNA barcodes were introduced to permit multiple samples per flowcell lane. 4C-libaries were prepared by first digesting nuclei with HindIII, followed by ligation and crosslink reversal. Chromatin was re-digested with NlaIII and re-ligated to generate DNA libraries by PCR using the Expand Long Template Polymerase Kit from Roche.