GSM4097048: 697 H3K27ac ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
697
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic
Attributes by original data submitter
Sample
source_name
pre-B leukemic 697 cells
cell line
pre-B leukemic cell line 697
treatment
parental 697
chip antibody
anti-H3K27ac (ab4729, Abcam)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNAs were extrated using Quick-RNA Miniprep kit (Zymo Research) . Chromatin lysates were prepared by sonication and protein-DNA complexes were isolated with indicated antibodies followed by purification with PCR purification kit (Qiagen). Multiplexing libraries were prepared for sequencing using Illumina standard protocol.