Antigen

Antigen Class

TFs and others

Antigen

Ctcf

Cell type

Cell type Class

Blood

Cell type

WEHI-231

Cell Type

B Lymphocyte, Immature

Disease

B Cell Lymphoma

Sample

source_name

Wehi-231-TFII-I KD

cell line

Wehi-231

tissue

B cell lymphoma

strain

(BALB/c x NZB)F1

chip antibody

CTCF Millipore #07-729

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and CTCF-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). Libraries were sequenced on the Illumina Hiseq 2000 following the manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

18239335

Reads aligned (%)

90.7

Duplicates removed (%)

41.0

Number of peaks

2394 (qval < 1E-05)

mm9

Number of total reads

18239335

Reads aligned (%)

90.5

Duplicates removed (%)

41.1

Number of peaks

2346 (qval < 1E-05)