Antigen

Antigen Class

TFs and others

Antigen

WDR5

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

cultured cell line

cell line

HEK293 cells

chip antibody

WDR5 antibody (Bethyl A302-430A)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After crosslinking and quenching of formaldehyde, lysates were prepared, sonicated to give a mean chromatin length of 200 bp, cleared by centrifugation, and cleared lysates subjected to reaction with antibodies (or IgG control) overnight. ChIP DNA was converted into an Illumina sequencing library using the NEBNext Ultra DNA Library Prep Kit (for Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

48483215

Reads aligned (%)

82.0

Duplicates removed (%)

6.8

Number of peaks

21630 (qval < 1E-05)

hg19

Number of total reads

48483215

Reads aligned (%)

81.7

Duplicates removed (%)

7.7

Number of peaks

21594 (qval < 1E-05)