Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
WDR5

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
cultured cell line
cell line
HEK293 cells
chip antibody
WDR5 antibody (Bethyl A302-430A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After crosslinking and quenching of formaldehyde, lysates were prepared, sonicated to give a mean chromatin length of 200 bp, cleared by centrifugation, and cleared lysates subjected to reaction with antibodies (or IgG control) overnight. ChIP DNA was converted into an Illumina sequencing library using the NEBNext Ultra DNA Library Prep Kit (for Illumina)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
48483215
Reads aligned (%)
75.3
Duplicates removed (%)
7.7
Number of peaks
21594 (qval < 1E-05)

hg38

Number of total reads
48483215
Reads aligned (%)
76.4
Duplicates removed (%)
6.8
Number of peaks
21630 (qval < 1E-05)

Base call quality data from DBCLS SRA