Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CREB5

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
metastatic lymph node lesion of human prostate cancer
cell type
metastatic human prostate cancer cell
chip antibody
CREB5_V5 (Cell signaling, #13202, lot2 )
cell line
LNCaP
treatment
DMSO for 4 days
expression
LNCaP expressing Luciferase

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIPseq, Lysates were sonicated,enriched with antibody for ChIP, decrosslinked, purified and prepped as library for the subsequent sequencing. For ATACseq, the purified intact nuclei were treated Transposase, extracted and prepped as library for further sequencing. For RNAseq, extracted RNA using collected from the cells using RNeasy plus kit (74104, Qiagen) was prepped as library for RNA sequencing. For ChIP seq, samples were prepared as library using using the ThruPLEX-FD Prep Kit (Rubicon Genomics, Ann Arbor, MI) with Illumina indexes. For ATACseq, TN5 (Nextera DNA Library Prep Kit) treated samples were amplified with index_primers and prepped as library.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
36184762
Reads aligned (%)
79.7
Duplicates removed (%)
14.9
Number of peaks
2091 (qval < 1E-05)

hg38

Number of total reads
36184762
Reads aligned (%)
82.0
Duplicates removed (%)
13.4
Number of peaks
2082 (qval < 1E-05)

Base call quality data from DBCLS SRA