metastatic lymph node lesion of human prostate cancer
metastatic human prostate cancer cell
Androgen Receptor ( Santa Cruz , sc-816x, lot J216)
2.5 uM enzalutamide for 4 days
LNCaP expressing Luciferase
Sequenced DNA Library
For ChIPseq, Lysates were sonicated,enriched with antibody for ChIP, decrosslinked, purified and prepped as library for the subsequent sequencing. For ATACseq, the purified intact nuclei were treated Transposase, extracted and prepped as library for further sequencing. For RNAseq, extracted RNA using collected from the cells using RNeasy plus kit (74104, Qiagen) was prepped as library for RNA sequencing. For ChIP seq, samples were prepared as library using using the ThruPLEX-FD Prep Kit (Rubicon Genomics, Ann Arbor, MI) with Illumina indexes. For ATACseq, TN5 (Nextera DNA Library Prep Kit) treated samples were amplified with index_primers and prepped as library.