Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTCF

Cell type

Cell type Class
Lung
Cell type
IMR-90
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
IMR90 lung fibroblasts
cell line
IMR90 lung fibroblasts
chip antibody
CTCF
treatment
20nM CTCF siRNA pool
time
144hrs

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were harvested according to Active Motif Low-Cell ChIP-seq kit (Cat no. 53084) Libraries were prepared according to Active Motif Low-Cell ChIP-seq kit (Cat no. 53084) Low Cell ChIP-seq

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
25752762
Reads aligned (%)
190.3
Duplicates removed (%)
1.9
Number of peaks
46 (qval < 1E-05)

hg38

Number of total reads
25752762
Reads aligned (%)
191.2
Duplicates removed (%)
1.9
Number of peaks
60 (qval < 1E-05)

Base call quality data from DBCLS SRA