Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP63

Cell type

Cell type Class
Epidermis
Cell type
HaCaT
Primary Tissue
Skin
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HaCaT
cell type
Human immortalized keratinocyte (HaCaT)
adenoviral infection
Ad-caRAS
histology
epidermal keratinocyte
ChIP
anti-p63a (clone H-129, Santa Cruz Biotechnology, lot number not available)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP were performed as described (Koinuma et al, Mol Cell Biol 29: 172-186, 2009). p63-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
28359721
Reads aligned (%)
84.7
Duplicates removed (%)
4.2
Number of peaks
7589 (qval < 1E-05)

hg38

Number of total reads
28359721
Reads aligned (%)
86.4
Duplicates removed (%)
3.2
Number of peaks
7479 (qval < 1E-05)

Base call quality data from DBCLS SRA