Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
DEK

Cell type

Cell type Class
Blood
Cell type
U-937
Primary Tissue
Blood
Tissue Diagnosis
Lymphoma Diffuse Large B Cell

Attributes by original data submitter

Sample

source_name
U937 cell line
chip antibody
DEK (Abcam, ab-74975)
cell line
U937

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed on U937 cells using the Magna-ChIP A/G Chromatin Immunoprecipitation Kit (Merck Millipore) after crosslinking with 1% formaldehyde for 15 min. Chromatin shearing was achieved by sonication in a Bioruptor UCD-200 (Diagenode). RNA libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
38193518
Reads aligned (%)
177.4
Duplicates removed (%)
7.5
Number of peaks
5241 (qval < 1E-05)

hg38

Number of total reads
38193518
Reads aligned (%)
178.8
Duplicates removed (%)
7.4
Number of peaks
5843 (qval < 1E-05)

Base call quality data from DBCLS SRA