Antigen

Antigen Class

TFs and others

Antigen

MITF

Cell type

Cell type Class

Epidermis

Cell type

Melanoma

MeSH Description

A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)

Sample

source_name

low passage primary melanoma cultures

cell type

low passage primary melanoma cultures

antibody

anti-MITF (ab12039, abcam)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was extracted using the MagnaChIP-seq kit (millipore). Sonication was performed for 12 cycles, 30 sec pulses using the biorupter Illumina Truseq

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

61894857

Reads aligned (%)

99.6

Duplicates removed (%)

94.3

Number of peaks

131 (qval < 1E-05)

hg19

Number of total reads

61894857

Reads aligned (%)

99.5

Duplicates removed (%)

94.6

Number of peaks

132 (qval < 1E-05)