SRX685339
GSM1484315: SKMEL5 Me3; Homo sapiens; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Epidermis
Cell type
SK-MEL-5
Primary Tissue
Skin
Tissue Diagnosis
Melanoma

Attributes by original data submitter

Sample

source_name
melanoma cell line
cell type
melanoma cell line
cell line
SKMEL5
antibody
H3K27me3 (07-499, Millipore)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was extracted using the MagnaChIP-seq kit (millipore). Sonication was performed for 12 cycles, 30 sec pulses using the biorupter Illumina Truseq

Sequencing Platform

instrument_model
Illumina HiSeq 2000

Where can I get the processing logs?

Read processing pipeline log

hg38

Number of total reads
28284455
Reads aligned (%)
98.3
Duplicates removed (%)
12.0
Number of peaks
3426 (qval < 1E-05)

hg19

Number of total reads
28284455
Reads aligned (%)
98.1
Duplicates removed (%)
12.6
Number of peaks
645 (qval < 1E-05)

Base call quality data from DBCLS SRA