RNA-seq: hTERT-RPE1 cells were collected in Trizol reagent, extracted with chloroform and precipitated with isopropanol. DNaseI treated RNA samples were then extracted using RNA clean & concentrator kit, Zymo Research. ChIP-seq: hTERT-RPE1 cells were washed once with PBS and crosslinked with 1% Formaldehyde for 10 min at room temparature for ChIP experiments. Chromatin was extracted in 1% SDS containg lysis buffer and sonicated. Cut&Run: hTERT-RPE1 cells were washed once with PBS and crosslinked with 1% Formaldehyde for 10 min at room temperature and Cut&Run protocol was performed as described in Skene PJ et al., 2017. Library is constructed as described before (Bowman et al., 2013 - Bowman SK, Simon MD, Deaton AM, Tolstorukov M, Borowsky ML, Kingston RE. Multiplexed Illumina sequencing libraries from picogram quantities of DNA. BMC Genomics. 2013;14:466. Published 2013 Jul 9. doi:10.1186/1471-2164-14-466).