Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived mesodermal cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mesoderm progenitors
cell type
hES-derived mesoderm progenitor cells
cell line
H9
chip antibody
none (input)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed as previously described (Brown et al., 2011, Stem Cells, 29(8): 1176-1185), with minor modifications. Briefly, cell lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with specific antibodies. Sequencing libraries were prepared using the ChIP-seq DNA sample kit (Illumina, IP-102-1001) with minor modifications and sequenced with a Genome Analyzer II (Illumina).

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
37211994
Reads aligned (%)
99.2
Duplicates removed (%)
26.6
Number of peaks
614 (qval < 1E-05)

hg19

Number of total reads
37211994
Reads aligned (%)
98.7
Duplicates removed (%)
27.1
Number of peaks
843 (qval < 1E-05)

Base call quality data from DBCLS SRA