GSM1483195: BRA FLyB Input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC derived mesodermal cells
NA
NA
Attributes by original data submitter
Sample
source_name
Mesoderm progenitors
cell type
hES-derived mesoderm progenitor cells
cell line
H9
chip antibody
none (input)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed as previously described (Brown et al., 2011, Stem Cells, 29(8): 1176-1185), with minor modifications. Briefly, cell lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with specific antibodies. Sequencing libraries were prepared using the ChIP-seq DNA sample kit (Illumina, IP-102-1001) with minor modifications and sequenced with a Genome Analyzer II (Illumina).