GSM4075263: H3K4me2 MP1 S6; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Cell type Class
Attributes by original data submitter
Human skin melanoma
Sequenced DNA Library
AMPure XP beads ChIP libraries were prepared according to illumina Genomic DNA protocol. Briefly, DNA fragments after ChIP were end repaired using two enzyme ( T4 DNA polymerase, T4 PNK), the blunted fragments were then A-tailed using Klenow fragment ( 3'-5- exo-) enzyme, Illumina adaptor ligation to the DNA fragments was carried on using T4 DNA Ligase from NEB (M0202), Libraries were then amplified for 8 cycles and size selected for an average of 350 bp, Libraries were then sequenced on NextSeq 500 following the manufacturer instruction.