GSM4075262: H3K4me2 MWN1 S3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Epidermis
Cell type
A-375
Primary Tissue
Skin
Tissue Diagnosis
Melanoma
Attributes by original data submitter
Sample
source_name
Human skin melanoma
cell type
A375 negative
passage
P8-P12
aggressiveness sorting
5 time
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
AMPure XP beads ChIP libraries were prepared according to illumina Genomic DNA protocol. Briefly, DNA fragments after ChIP were end repaired using two enzyme ( T4 DNA polymerase, T4 PNK), the blunted fragments were then A-tailed using Klenow fragment ( 3'-5- exo-) enzyme, Illumina adaptor ligation to the DNA fragments was carried on using T4 DNA Ligase from NEB (M0202), Libraries were then amplified for 8 cycles and size selected for an average of 350 bp, Libraries were then sequenced on NextSeq 500 following the manufacturer instruction.