Antigen

Antigen Class

TFs and others

Antigen

TEAD4

Cell type

Cell type Class

Breast

Cell type

MCF 10A

Primary Tissue

Breast

Tissue Diagnosis

Fibrocystic Disease

Sample

source_name

Mammary Epthelial Cells

tissue

Normal Immortalized MCF10A Mammary Epithelial Cells

passage

10-15

antibody

sc-101184

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and Antibody-DNA complexes were isolated with antibody. Libraries were prepared according to ActiveMotif's Low Cell ChIP-seq Kit instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

69076694

Reads aligned (%)

95.8

Duplicates removed (%)

17.8

Number of peaks

9625 (qval < 1E-05)

hg19

Number of total reads

69076694

Reads aligned (%)

95.4

Duplicates removed (%)

17.8

Number of peaks

9349 (qval < 1E-05)