Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMARCA4

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa
cell type
Cervical cancer cells
cell line
HeLa
chip antibody
BRG1 (Abcam Catalog# B8184)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq library was prepared as per the instructions of user manual of NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (Catalog # E7370) with certain optimization and initial 100 ng of DNA purified after ChIP.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
45207300
Reads aligned (%)
1.8
Duplicates removed (%)
6.8
Number of peaks
120 (qval < 1E-05)

hg38

Number of total reads
45207300
Reads aligned (%)
1.9
Duplicates removed (%)
6.6
Number of peaks
142 (qval < 1E-05)

Base call quality data from DBCLS SRA