Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Larvae
Cell type
L2-4

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
whole cell
cell line
L2-4 Flag-HA-HMR/pMT Hygro+Myc-LHR/pMT Hygro
treatment
HMR+LHR induced
ChIP
input

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChiP is prefromed exactly as described in Gerland et al, 2017. Shortly, the cells were crosslinked in 1% FA for 5 min at room temperature and frozen. Chromatin from 50 million cells was sheared in TE/0.1%SDS, the buffer was adjusted to RIPA and after preclearing the sheared extract and taking 1/10 as an input, IP was done overnight. Beads were washed, after which RNA/protein digestion and decrosslinking was performed. DNA was purified using AMPure XP beads. Libraries were prepared from 1 ng DNA with Microplex Diagenode kit, according to manufacture's instructions, without size selection.

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
25845210
Reads aligned (%)
96.9
Duplicates removed (%)
17.1
Number of peaks
3493 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA