Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RUNX1

Cell type

Cell type Class
Blood
Cell type
TSU-1621MT
NA
NA

Attributes by original data submitter

Sample

source_name
TSU-1621-MT_ChIP RUNX1
cell line
TSU-1621-MT
antibody target
RUNX1
chip antibody vendor
Abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was sonicated and used for ChIP seq using specific antibodies. RNA was extracted using qiagen RNeasy kit according to manufacturer instructions and total RNA was treated using Ribo-zero to remove rRNA according to manufacturer instructions. ChIP seq and RNA-seq libraries were prepared according to Illumina's standard protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
37278785
Reads aligned (%)
76.9
Duplicates removed (%)
21.5
Number of peaks
52116 (qval < 1E-05)

hg38

Number of total reads
37278785
Reads aligned (%)
78.2
Duplicates removed (%)
20.6
Number of peaks
52211 (qval < 1E-05)

Base call quality data from DBCLS SRA