Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

TSU-1621MT

NA

NA

Sample

source_name

TSU-1621-MT_ChIP CTD of RNA polymerase II ATRA

cell line

TSU-1621-MT

treated with

1 uM ATRA for 24 hrs

antibody target

RNAPII

chip antibody vendor

Diagenode

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was sonicated and used for ChIP seq using specific antibodies. RNA was extracted using qiagen RNeasy kit according to manufacturer instructions and total RNA was treated using Ribo-zero to remove rRNA according to manufacturer instructions. ChIP seq and RNA-seq libraries were prepared according to Illumina's standard protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

44366039

Reads aligned (%)

91.6

Duplicates removed (%)

10.7

Number of peaks

23014 (qval < 1E-05)

hg19

Number of total reads

44366039

Reads aligned (%)

90.8

Duplicates removed (%)

11.7

Number of peaks

23044 (qval < 1E-05)