Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

NB-4

Primary Tissue

Blood

Site of Extraction

Bone Marrow

Tissue Diagnosis

Leukemia

Sample

source_name

NB4 cell line

cell line

NB4

cell type

Leukemic cells

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was fixed in 1% formaldehyde for 10 minutes, sheared to 200-700bp using a Bioruptor Diagenode, immunoprecipitated using the indicated antibody, purified and sequenced using Illumina Genome Analyzers as recommended by the manufacturer. Libraries were created from successful large-scaled ChIP experiments using Illumina's TruSeq Library Prep Kit following instructions in the user manual (Illumina, San Diego, CA, USA). The library size was estimated using a Bioanalyzer 2100

Sequencing Platform

instrument_model

HiSeq X Ten

hg38

Number of total reads

28898340

Reads aligned (%)

91.3

Duplicates removed (%)

15.3

Number of peaks

1123 (qval < 1E-05)

hg19

Number of total reads

28898340

Reads aligned (%)

90.0

Duplicates removed (%)

15.6

Number of peaks

646 (qval < 1E-05)