GSM4066998: shNC H3K9me2 ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K9me2
Cell type
Cell type Class
Blood
Cell type
NB-4
Primary Tissue
Blood
Site of Extraction
Bone Marrow
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
NB4 cell line
cell line
NB4
cell type
Leukemic cells
chip antibody
H3K9me2 (Active Motif, 39753,lot 06710001)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was fixed in 1% formaldehyde for 10 minutes, sheared to 200-700bp using a Bioruptor Diagenode, immunoprecipitated using the indicated antibody, purified and sequenced using Illumina Genome Analyzers as recommended by the manufacturer. Libraries were created from successful large-scaled ChIP experiments using Illumina's TruSeq Library Prep Kit following instructions in the user manual (Illumina, San Diego, CA, USA). The library size was estimated using a Bioanalyzer 2100