Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Embryo
Cell type
4-12h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
D. Melanogaster_H3K4me3
genotype/variation
wild type
tissue
embryos
age
4-12 hrs
chip antibody
H3K4me3, Millipore #07-473

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin prepared from Drosophila embryos was immunoprecipitated with specific antibodies and precipitated DNA was isolated (see mat and methods for details) Libraries were prepared according to Illumina's instructions

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

dm6

Number of total reads
4205145
Reads aligned (%)
93.7
Duplicates removed (%)
49.6
Number of peaks
3204 (qval < 1E-05)

dm3

Number of total reads
4205145
Reads aligned (%)
94.4
Duplicates removed (%)
48.1
Number of peaks
3010 (qval < 1E-05)

Base call quality data from DBCLS SRA