Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Cell line
Cell type
S2

Cell type information


Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by Original Data Submitter


source_name
S2 cells
cell line
S2
tissue
embryo cell line
chip antibody
none

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
S2 cells were cross-linked for 15 min at 37℃ in 1 ml of 1% formaldehyde in PBS buffer. The cross-linking was stopped by adding Glycine to a final concentration of 0.125M. Cells were sonicated with a Bioruptor sonicator to yield genomic DNA fragments with an average size of about 200 bp. protein-DNA complexes were isolated with Lola antibody. Genomic DNA was purified with a DNA purification kit (QIAGEN). Libraries were prepared using standard Illumina protocols.

Platform Information


instrument_model
Illumina HiSeq 2500

External Database Query

Logs in read processing pipeline


Number of total reads
28904776
Reads aligned (%)
96.9
Duplicates removed (%)
15.8
Number of peaks
3663 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA