Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
JUN

Cell type

Cell type Class
Blood
Cell type
CAR-T cells
NA
NA

Attributes by original data submitter

Sample

source_name
patient-derived CAR-T_JUN-HA
cell type
patient-derived CAR-T
genotype/variation
Retroviral transduction with JUN-HA construct
growth protocol
T cell media + IL-2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells are fixed with 1% FA. Following ChIP and washes, DNA is purified using Ampure XP End-repair and A-tailing performed with NEB Ultra II Kit per protocol. Adaptor ligation and indexing with NEBNext Multiplex Oligos

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
35517907
Reads aligned (%)
178.6
Duplicates removed (%)
8.8
Number of peaks
515 (qval < 1E-05)

hg38

Number of total reads
35517907
Reads aligned (%)
180.4
Duplicates removed (%)
8.5
Number of peaks
1005 (qval < 1E-05)

Base call quality data from DBCLS SRA