GSM4059679: JUN-HA, replicate 1 [jun-1-jun-CAGATC S6]; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
JUN
Cell type
Cell type Class
Blood
Cell type
CAR-T cells
NA
NA
Attributes by original data submitter
Sample
source_name
patient-derived CAR-T_JUN-HA
cell type
patient-derived CAR-T
genotype/variation
Retroviral transduction with JUN-HA construct
growth protocol
T cell media + IL-2
chip antibody
JUN (Active Motif, #39309)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells are fixed with 1% FA. Following ChIP and washes, DNA is purified using Ampure XP End-repair and A-tailing performed with NEB Ultra II Kit per protocol. Adaptor ligation and indexing with NEBNext Multiplex Oligos