Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
IRF4

Cell type

Cell type Class
Blood
Cell type
CAR-T cells
NA
NA

Attributes by original data submitter

Sample

source_name
patient-derived CAR-T_HA only
cell type
patient-derived CAR-T
genotype/variation
Retroviral transduction with HA construct
growth protocol
T cell media + IL-2
chip antibody
IRF4 (Abcam, #ab101168)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells are fixed with 1% FA. Following ChIP and washes, DNA is purified using Ampure XP End-repair and A-tailing performed with NEB Ultra II Kit per protocol. Adaptor ligation and indexing with NEBNext Multiplex Oligos

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
53464296
Reads aligned (%)
153.2
Duplicates removed (%)
19.3
Number of peaks
21970 (qval < 1E-05)

hg38

Number of total reads
53464296
Reads aligned (%)
155.5
Duplicates removed (%)
18.7
Number of peaks
23701 (qval < 1E-05)

Base call quality data from DBCLS SRA