Antigen

Antigen Class

Histone

Antigen

H2A.XS139ph

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

chronic myelogenous leukemia

cell line

K562

cell type

chronic myelogenous leukemia cell line

treated with

Topotecan 10mM for 4hr

chip antibody

anti-gamma-H2AX

chip antibody vendor

Millipore

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For FAIRE-seq, after fixation, lysates were cleared from sonicated nuclei and histone-free DNA were isolated by phenol-chloroform extraction.for ChIP samples. For ChIP-seq, after fixation, lysates were cleared from sonicated nuclei and antibodies against g-H2AX were used to IP the histone-DNA complexes. Libraries were prepared according to the provider’s kits and protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

37918792

Reads aligned (%)

97.8

Duplicates removed (%)

1.6

Number of peaks

1068 (qval < 1E-05)

hg19

Number of total reads

37918792

Reads aligned (%)

97.3

Duplicates removed (%)

2.6

Number of peaks

1083 (qval < 1E-05)