GSM1477632: K562 g-H2AX Daun 4hr 1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H2A.XS139ph
Cell type
Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous
Attributes by original data submitter
Sample
source_name
chronic myelogenous leukemia
cell line
K562
cell type
chronic myelogenous leukemia cell line
treated with
Daunorubucin 10mM for 4hr
chip antibody
anti-gamma-H2AX
chip antibody vendor
Millipore
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For FAIRE-seq, after fixation, lysates were cleared from sonicated nuclei and histone-free DNA were isolated by phenol-chloroform extraction.for ChIP samples. For ChIP-seq, after fixation, lysates were cleared from sonicated nuclei and antibodies against g-H2AX were used to IP the histone-DNA complexes. Libraries were prepared according to the provider’s kits and protocols.