Epithelial tumor cells isolated from human colorectal adenocarcinoma
goat anti–human PROX1 antibody (R&D Systems)
Sequenced DNA Library
0.5x107 SW480R cells were cross-linked with formaldehyde, chromatin was fragmented using Simple ChIP Enzymatic Chromatin IP Kits (Cell Signaling) and immunoprecipitated using 5µg goat anti–human PROX1 antibody (R&D Systems) complexed with protein G Sepharose (GE Healthcare). 10 ng of ChIP sample was quantified using the Qubit fluorometer (Invitrogen) and DNA were prepared using the ChIP-Seq Sample Preparation Kit (Illumina) according to the manufacturer’s protocol.