Antigen

Antigen Class

TFs and others

Antigen

Cas9

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

Chronic Myelogenous Leukemia

cell line

K562

sgrna

LTR2B

chip antibody

anti-Cas9 (Diagenode C15200229-100)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was extracted from cross-linked cells, and sonicated to 200-700 bp fragments. Immunoprecipitation was performed with 15 µg of chromatin for 2.5 µg of anti-H3K27ac and H3K9me3 antibody or 75µg chromatin for 5µg Cas9 antibody. DNA was purified using the GeneJET PCR purification kit (Thermo Fisher). Libraries were prepared from 1-5 ng of DNA using the NEBNext ultra II DNA library prep kit.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

166807135

Reads aligned (%)

87.2

Duplicates removed (%)

41.5

Number of peaks

4019 (qval < 1E-05)

hg19

Number of total reads

166807135

Reads aligned (%)

86.6

Duplicates removed (%)

43.3

Number of peaks

2692 (qval < 1E-05)