GSM4057510: K562 LTR2BgRNAs H3K27ac-1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous
Attributes by original data submitter
Sample
source_name
Chronic Myelogenous Leukemia
cell line
K562
sgrna
LTR2B
chip antibody
anti-H3K27ac (Active Motif 39133)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was extracted from cross-linked cells, and sonicated to 200-700 bp fragments. Immunoprecipitation was performed with 15 µg of chromatin for 2.5 µg of anti-H3K27ac and H3K9me3 antibody or 75µg chromatin for 5µg Cas9 antibody. DNA was purified using the GeneJET PCR purification kit (Thermo Fisher). Libraries were prepared from 1-5 ng of DNA using the NEBNext ultra II DNA library prep kit.