Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Blood

Cell type

HL-60

Primary Tissue

Blood

Tissue Diagnosis

Leukemia

Sample

source_name

Acute Myeloid Leukemia

cell line

HL-60

Sequenced DNA Library

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

Cells were resuspended in RSB buffer (10 mM NaCl, 3mM MgCl2, 10mM Tris-Cl, pH 7.4). After cell lysis, the nuclei were digested with DNase I with 15 U for 10 min at 37oC. Digests were inactivated by the addition of 50 mM EDTA. RNA and proteins were digestion by RNase A (0.5 mg/ml) for 15 min at at 37°C and then by proteinase K (0.5 mg/ml) for 1 h at 65 oC. DNA was purified by phenol-chloroform extraction and ethanol precipitation. Libraries were prepared from 1-5 ng of DNA using the NEBNext ultra II DNA library prep kit.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

18560632

Reads aligned (%)

81.0

Duplicates removed (%)

0.6

Number of peaks

463 (qval < 1E-05)

hg19

Number of total reads

18560632

Reads aligned (%)

80.5

Duplicates removed (%)

0.7

Number of peaks

356 (qval < 1E-05)