Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ELF1

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549 cells
cell line
A549
cell type
lung epithelial adenocarcinoma cell line
passages
18-25
chip antibody
anti-ELF1 antibody, Cat# sc-631; RRID:AB_631428;Clone: C-20

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and ELF1-DNA complexes were isolated with antibody. Input or ChIPped DNA was end-repaired and "A" bases were subsequently added to the DNA. After purification, Illumina/Solexa PE adapters were ligated to DNA fragments. The DNA fragments were then purified and subjected to PCR using Illumina/Solexa primers PE 1.0 and 2.0. These final ChIP-Seq libraries were then purified with the QIAGEN MinElute kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
223221549
Reads aligned (%)
49.5
Duplicates removed (%)
40.8
Number of peaks
16754 (qval < 1E-05)

hg38

Number of total reads
223221549
Reads aligned (%)
50.9
Duplicates removed (%)
39.4
Number of peaks
17581 (qval < 1E-05)

Base call quality data from DBCLS SRA