Lysates were clarified from sonicated nuclei and ELF1-DNA complexes were isolated with antibody. Input or ChIPped DNA was end-repaired and "A" bases were subsequently added to the DNA. After purification, Illumina/Solexa PE adapters were ligated to DNA fragments. The DNA fragments were then purified and subjected to PCR using Illumina/Solexa primers PE 1.0 and 2.0. These final ChIP-Seq libraries were then purified with the QIAGEN MinElute kit.